Well, first of all I don't think any of the tilset rippers or whatever support transparency. the easiest thing to do is just open a tile in the RPGTK editor. then go to "image" at the top drop down menu and select "grab tiles", when that opens there is a picture of a folder on the bottom toolbar. Click that open your image. then on the bottom toolbar agian click, "Batch Grab 32x32" after you click that you will be prompted to save it as a tileset. Tah Dah! also, if you do want transparency, then open each tile and fill in the transparent pixels with transparency. Tah Dah!